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Fluorescent Immunochromatographic Technique

Fluorescence immunochromatography is a new membrane detection technology based on antigen - antibody - specific immune response. The technique uses a strip of fibrous tomography material with a fixed detection line (coated antibody or coated antigen) and a quality control line (anti-antibody) as the stationary phase, the test fluid as the flowing phase, the fluorescent-labeled antibody or antigen fixed on the attachment pad, and the analyte is moved on the tomography strip by capillary action. For with multiple antigenic determinant of macromolecular antigen (protein, virus, bacteria, etc.), usually adopt "sandwich" type double sandwich immune chromatography method, namely the object under test mobile phase under the action of combined with fluorescently labeled antibodies, first when they arrive in line again when combined with package is antibodies to form a double layer resistant type "sandwich".

Immunochromatography

Immune chromatography technology (ImmunochromatographicAssay, ICA) is developed at the end of the 20th century combined with immune technology and an analysis method of chromatography chromatography technology, the method is specific, simple operation, fast and other characteristics, widely used in clinical diagnosis, environmental monitoring, food safety and other important fields. Traditional immunochromatography takes colloidal gold as the marker, and qualitative detection or semi-quantitative analysis of the target object is carried out by strip color rendering. Although this method is simple and fast, its sensitivity is poor and it is difficult to quantify accurately. As a new immunoassay technology, fluorescence immunochromatography not only retains the advantages of rapid field detection of traditional colloidal gold test strips, but also adds the high sensitivity of labor, light and thrifty measurement technology, which has become one of the main ways to improve the detection performance of immunochromatography methods.

Fluorescence immunochromatography

 Fluorescence immunochromatography is a new membrane detection technology based on antigen-antibody - specific immune response. The technique USES a strip of fibrous tomography material with a fixed detection line (coated antibody or coated antigen) and a quality control line (anti-antibody) as the stationary phase, the test fluid as the flowing phase, the fluorescent-labeled antibody or antigen fixed on the attachment pad, and the analyte is moved on the tomography strip by capillary action. For with multiple antigenic determinant of macromolecular antigen (protein, virus, bacteria, etc.), usually adopt "sandwich" type double sandwich immune chromatography method, namely the object under test mobile phase under the action of combined with fluorescently labeled antibodies, first when they arrive in line again when combined with package is antibodies to form a double layer resistant type "sandwich". For small molecular antigens with only one epitope (agro-veterinary drugs, prohibited drugs, etc.), it is difficult to combine the detected small molecular antigen with the fluorescent-labeled antibody again due to the steric hindrance. Therefore, small molecules with single antigen epitopes are mostly detected by competitive immunochromatography.

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