Fluorescence immunochromatography has the advantages of high sensitivity, good stability and low interference from natural fluorescence, which has become the focus of fast food safety detection and analysis. At present, the markers used for fluorescence immunoassay mainly include fluorescein, quantum dots, up-conversion nanoparticles and so on.
Fluorescein immunofluorescence chromatography
Fluorescein refers to organic dyes with fluorescent properties. In 1942, Coons et al. first reported the study on the distribution of pneumococcal antigen in mouse tissue sections using fluorescein isocyanate labeled antibody, and thus developed immunofluorescence technology. Later, in order to improve the performance of isocyanate fluorescein, Ruggas et al. synthesized isothiocyanate fluorescein with stable properties and low toxicity. Since Marshall et al. improved fluorescence antibody labeling technology, fluorescence immunity technology has been developed rapidly. At present, there are 5 kinds of fluorescein used in fluorescence immunoassay: isothiocyanate fluorescein, tetraethylene-rhodamine, tetramethyliso-thiocyanate rhodamine, fluorescein substance and lanthanide system after enzyme action.
Song chunmei et al. used isothiocyanate moxibustion photoelement as the marker material to mark the monoclonal antibody of ractopamine. The results showed that the detection limit of the immunohistochromatography paper strip was 1. The results of cross reaction with structural analogues show that this method has good specificity. The ledodopa residue in milk and other samples was detected with different batches of test paper, with high consistency and good reproducibility .
The application of fluorescent materials in immunochromatography has greatly improved the detection performance of this method. However, organic dye markers are easy to decompose and bleach under light, and have the characteristics of concentration quenching, which affects the accuracy and reliability of the analysis results. Fluorescein encapsulated in polystyrene microspheres is an effective method to improve the performance of fluorescein analysis.
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