The ovulation test is designed to predict ovulation by detecting the peak level of luteinizing hormone (LH). It can be used for qualitative detection of luteinizing hormone (LH) in human urine, so as to determine the "safe period" of ovulation time and women's menstrual cycle, so as to select the best time for conception or use "safe period" contraception.
Ovulation test paper is an in vitro test and auxiliary diagnosis of ovulation in women. Ovulation test paper is suitable for about 30% of women, and is only used as an auxiliary means to detect ovulation in the clinic.
It is made up by use immunochromatographic double-antibody clamping heart method principle and colloidal gold label technology, which is prepared by sequentially attaching a mouse anti-a-lh monoclonal antibody (detection line) and goat anti-mouse IgG polyclonal antibody (control line), and that other adjuvants of the monoclonal antibody against beta-lh monoclonal antibody are sequentially attached.
In detection, the LH in urine sample is combined with the pre-solid beta-lh monoclonal antibody colloidal gold of the sample pad end of the test paper, and the bond migrates upwards to the detection line (T) region along the capillary action of the membrane, and reacts with the a-lh antibody on the membrane, and a red strip appears in the region, whether or not there is LH in the specimen, and when the liquid level continues to migrate to the control line (C) region, a red band is bound to appear in the region. If the color of the red stripe in the detection line area (T) is darker or similar than that of the red stripe in the control line area (C), it indicates a positive result, meaning that the subject is in ovulation. If the color of the red stripe in the test line area (T) is lighter than that in the control line area (C), it indicates a negative result, meaning that the subject is not ovulating. The red stripe shown in the control line area (C) is the standard to judge whether there are enough specimens, whether the chromatography process is normal, and also serves as the internal control standard of reagents.
According to the principle of ovulation test paper, we find out the most suitable method for ovulation test. That is, once a week after the end of menstruation, we use ovulation test paper every day. If the contrast test paper finds that the ovulation test paper begins to weaken, and quickly weakens, then the ovulation test paper is about to be released, and the room can be arranged. The time range of ovulation is usually within 24-48 hours after the weakness of the ovulatory test paper.
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