Shanghai Chemtron Biotech Co.Ltd.

Up-Conversion Nanoparticles Immunochromatographic Technique

Up-conversion luminescence refers to back-stokes luminescence, which USES low-energy infrared or near-infrared light to trigger the emission of high-energy ultraviolet or visible light. Most up-conversion luminescence occurs in compounds doped with rare earth ions. NaYF2 is the substrate material with the highest efficiency of up-conversion luminescence. Compared with fluorescent dyes and quantum dots, up-conversion nanoparticles have low toxicity, high sensitivity and good stability, which can avoid the influence of fluorescence matrix on the test results, and are one of the ideal fluorescent markers at present.

Based on the conversion immunofluorescence on single target test, Adrian wong and others developed salmonella paratyphi b, 0139 group of vibrio cholerae O1, e. coli O157, salmonella paratyphi a and salmonella enteritidis, pigs cholera salmonella and other 10 kinds of food-borne pathogens with ten channels of the on-site detection test paper plate detection system, which can meet the requirements of food sample detection sensitivity and diarrhea sample. Liu xiao et al., with the above converted luminescent materials as markers, established a fast up-conversion immunochromatography quantitative detection method for on-site rapid detection of aflatoxin M1 in dairy products. Under optimal conditions, the sensitivity of this method to aflatoxin M1 in milk powder and milk reached 0.1ng/mL and 0.3ng/mL respectively, and the variation coefficient of repeated measurement at each concentration was less than 15%. The up-conversion fluorescence immunochromatography paper strip detection method constructed in this study is simple and fast in operation, simple in sample pretreatment, has good detection sensitivity and specificity, has good tolerance to actual samples, and can meet the need of rapid on-site detection in food safety.

Liu and others use the core-shell type NaGdF4: Yb nanoparticles and physicochemical antibody coupling as a fluorescent probe, spraying in combination of pad, with physicochemical - coupling and bovine serum albumin sheep spray resistance against mouse antibody respectively on the line of nitrocellulose membrane as the line and quality control. Under optimal conditions, the established up-conversion fluorescence immunochromatography method has a visual detection line of 10ng/mL and a linear range of 0.5-100ng/mL for quantitative detection

Chemtron biotech rapid test reader is of high quality. Learn more about the rapid test reader from chemtron biotech.
Related News
  • Urinary Microalbumin and Nephropathy

    Urinary Microalbumin and Nephropathy

    August 8, 2018Proteinuria is the main clinical symptom of nephropathy, and the detection of microalbumin is of great clinical value in determining the degree of disease and prognosis. Because the main symptom of ne...view
  • Urinary Microalbumin and Cardiovascular Disease

    Urinary Microalbumin and Cardiovascular Disease

    August 8, 2018Through the study of diabetic patients, it was found that the incidence of cardiovascular diseases in MUA positive patients was higher, the onset time was earlier, and the degree of lesions was more s...view
  • Fluorescent Immunochromatographic Technique

    Fluorescent Immunochromatographic Technique

    August 8, 2018Fluorescence immunochromatography is a new membrane detection technology based on antigen - antibody - specific immune response. The technique uses a strip of fibrous tomography material with a fixed ...view
  • The Influence of FSH on Test-tube Baby

    The Influence of FSH on Test-tube Baby

    August 8, 2018Prior to ivf, the reproductive center will require couples to perform a complete pre-operative examination of ivf, including six assays of sex hormones (FSH, LH, E2, P, T, PRL), requiring bleeding fro...view
  • Interpretation of FSH

    Interpretation of FSH

    August 8, 2018FSH is a hormone secreted by basophils in the anterior pituitary gland. It is composed of glycoproteins and its main function is to promote follicular maturation. Human follicular stimulating hormone ...view
  • Several Methods For Detection Of Salmonella

    Several Methods For Detection Of Salmonella

    August 8, 2018Salmonella is a pathogenic bacterium, so in our country, its limit in food is 0, once detected, it is an unqualified product, not allowed to enter the market.1. Traditional national standard method of...view
  • TEL:+86-21-68129666
  • FAX:+86-21-68129222
  • EMAIL:international@chemtronbio.com
  • ADDRESS:No.518, Qingdai Rd., International Medical Park, Pudong, 201318, Shanghai, P.R.China