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Up-Conversion Nanoparticles Immunochromatographic Technique

Up-conversion luminescence refers to back-stokes luminescence, which USES low-energy infrared or near-infrared light to trigger the emission of high-energy ultraviolet or visible light. Most up-conversion luminescence occurs in compounds doped with rare earth ions. NaYF2 is the substrate material with the highest efficiency of up-conversion luminescence. Compared with fluorescent dyes and quantum dots, up-conversion nanoparticles have low toxicity, high sensitivity and good stability, which can avoid the influence of fluorescence matrix on the test results, and are one of the ideal fluorescent markers at present.

Based on the conversion immunofluorescence on single target test, Adrian wong and others developed salmonella paratyphi b, 0139 group of vibrio cholerae O1, e. coli O157, salmonella paratyphi a and salmonella enteritidis, pigs cholera salmonella and other 10 kinds of food-borne pathogens with ten channels of the on-site detection test paper plate detection system, which can meet the requirements of food sample detection sensitivity and diarrhea sample. Liu xiao et al., with the above converted luminescent materials as markers, established a fast up-conversion immunochromatography quantitative detection method for on-site rapid detection of aflatoxin M1 in dairy products. Under optimal conditions, the sensitivity of this method to aflatoxin M1 in milk powder and milk reached 0.1ng/mL and 0.3ng/mL respectively, and the variation coefficient of repeated measurement at each concentration was less than 15%. The up-conversion fluorescence immunochromatography paper strip detection method constructed in this study is simple and fast in operation, simple in sample pretreatment, has good detection sensitivity and specificity, has good tolerance to actual samples, and can meet the need of rapid on-site detection in food safety.

Liu and others use the core-shell type NaGdF4: Yb nanoparticles and physicochemical antibody coupling as a fluorescent probe, spraying in combination of pad, with physicochemical - coupling and bovine serum albumin sheep spray resistance against mouse antibody respectively on the line of nitrocellulose membrane as the line and quality control. Under optimal conditions, the established up-conversion fluorescence immunochromatography method has a visual detection line of 10ng/mL and a linear range of 0.5-100ng/mL for quantitative detection

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